Two complete squence primers were designed based on the result of race . a 1475bp sequence was amplified by pcr . the analysis of the product shows that the nucleotide and deduced amino acid sequences share 40 % - 60 % homologous to the corresponding parts of - glc gene family of pinus contorta , cucurbita pepo , arabidopsis thaliana by the blast _ w program comparison 經blast搜索表明:克隆所得堿基序列和推導的氨基酸序列與已克隆出的小松樹、西葫蘆擬南芥等植物體內-葡萄糖苷酶基因的cdna相應序列有40 ? 60的同源性,因此我們推斷擴增所得到的序列為茶樹中-葡萄糖苷酶基因的cdna 。